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GfaABC1D BARK D110A p2A mCherry
(Plasmid #117692)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117692 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZac2.1
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BARKrgs D110A peptide
  • Alt name
    GfaABC1D-iBARK(D110A)-p2A-mCherry
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    1254
  • Mutation
    D110A
  • Tag / Fusion Protein
    • HA / FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GfaABC1D BARK D110A p2A mCherry was a gift from Baljit Khakh (Addgene plasmid # 117692 ; http://n2t.net/addgene:117692 ; RRID:Addgene_117692)

  • For your References section:

    Specific and behaviorally consequential astrocyte Gq GPCR signaling attenuation in vivo with ibetaARK. Nagai J, Bellafard A, Qu Z, Yu X, Ollivier M, Gangwani MR, Diaz-Castro B, Coppola G, Schumacher SM, Golshani P, Gradinaru V, Khakh BS. Neuron. 2021 Jul 21;109(14):2256-2274.e9. doi: 10.1016/j.neuron.2021.05.023. Epub 2021 Jun 16. 10.1016/j.neuron.2021.05.023 PubMed 34139149
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