pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher
(Plasmid #117656)

• Purpose: Bacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.
• Depositing Lab: Bo Huang
• Publication: Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.1038/s42003-019-0589-x. eCollection 2019.

Backbone

• Vector backbone: pETDuet-1
• Backbone size w/o insert (bp): 5420
• Total vector size (bp): 6551
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: sfCherry2(1-10)
• Species: Synthetic
• Insert Size (bp): 621
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AGGAGATATACCATGGAGGAGGACAACATGG
• 3′ sequencing primer: TGGCTGCTGCCCATGTCAGTCCTCGTTGTGGCTGGTGA

Gene/Insert 2

• Gene/Insert name: sfCherry2(11)-SpyCatcher002
• Species: Synthetic
• Insert Size (bp): 498
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AAGGAGATATACATAATGTACACCATCGTGGAGCAG
• 3′ sequencing primer: TTGAGATCTGCCATAttaaatatgagcgtcacctttagttgctttgcca

Resource Information

• Supplemental Documents:
  • pETDuet_sfCherry2(1-10)_sfCherry2(11)-SPC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/454041v1 for BioRxiv preprint