pCMV5B-TGFbeta receptor I K232R
(Plasmid #11763)

• Depositing Lab: Jeff Wrana
• Publication: Wieser et al EMBO J. 1995 May 15. 14(10):2199-208.

Backbone

• Vector backbone: pCMV5B
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TGFbeta receptor I
• Species: H. sapiens (human)
• Insert Size (bp): 1600
• Mutation: K232R: kinase dead
• GenBank ID: NM_004612
• Entrez Gene: TGFBR1 (a.k.a. AAT5, ACVRLK4, ALK-5, ALK5, ESS1, LDS1, LDS1A, LDS2A, MSSE, SKR4, TBR-i, TBRI, TGFR-1, tbetaR-I)
• Promoter: CMV
• Tag / Fusion Protein:
  • 6xHIS (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGHpA-R

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA