pCMV5B-TFGbeta receptor II K277R
(Plasmid #11762)

• Depositing Labs: Joan Massague, Jeff Wrana
• Publication: Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

Backbone

• Vector backbone: pCMV5B
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TGFbeta receptor II
• Species: H. sapiens (human)
• Insert Size (bp): 1800
• Mutation: K277R: kinase dead
• GenBank ID: NM_003242
• Entrez Gene: TGFBR2 (a.k.a. AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TBR-ii, TBRII, TGFR-2, TGFbeta-RII, tbetaR-II)
• Promoter: CMV
• Tag / Fusion Protein:
  • His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGHpA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid was constructed at the Massague lab at MSKCC. Please acknowledge both the Wrana and Massague labs in your publication.
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA