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Addgene

RNQ pROEX-HTb
(Plasmid #1176)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1176 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPROEX-HTb
  • Backbone manufacturer
    Gibco-BRL
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RNQ1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1218
  • Entrez Gene
    RNQ1 (a.k.a. YCL028W)
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • TEV cleavage site (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HIndIII (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RNQ pROEX-HTb was a gift from Susan Lindquist (Addgene plasmid # 1176 ; http://n2t.net/addgene:1176 ; RRID:Addgene_1176)

  • For your References section:

    Rnq1: an epigenetic modifier of protein function in yeast. Sondheimer N, Lindquist S. Mol Cell 2000 Jan;5(1):163-72. 10.1016/S1097-2765(00)80412-8 PubMed 10678178
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