pCMV5B-ALK4
(Plasmid #11756)

• Depositing Lab: Jeff Wrana
• Publication: Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-36.

Backbone

• Vector backbone: pCMV5B
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ALK4
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• GenBank ID: NM_020328
• Entrez Gene: ACVR1B (a.k.a. ACTRIB, ACVRLK4, ALK4, SKR2)
• Promoter: CMV
• Tag / Fusion Protein:
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGH-PA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA