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Addgene

pCMV5-ALK2 K235R
(Plasmid #11755)

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Item Catalog # Description Quantity Price (USD)
Plasmid 11755 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pCMV5
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ALK2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Mutation
    K235R: kinase dead
  • GenBank ID
    NM_001105
  • Entrez Gene
    ACVR1 (a.k.a. ACTRI, ACVR1A, ACVRLK2, ALK2, FOP, SKR1, TSRI)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5-ALK2 K235R was a gift from Jeff Wrana (Addgene plasmid # 11755 ; http://n2t.net/addgene:11755 ; RRID:Addgene_11755)

  • For your References section:

    Specific activation of Smad1 signaling pathways by the BMP7 type I receptor, ALK2. Macias-Silva M, Hoodless PA, Tang SJ, Buchwald M, Wrana JL. J Biol Chem. 1998 Oct 2. 273(40):25628-36. 10.1074/jbc.273.40.25628 PubMed 9748228
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