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pCMV5B-Flag-Smurf1 wt
(Plasmid #11752)

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Item Catalog # Description Quantity Price (USD)
Plasmid 11752 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pCMV5B
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SMURF1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2300
  • GenBank ID
    NM_020429
  • Entrez Gene
    SMURF1
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A human Smurf1 cDNA encoding all but the first eight amino acids was identified in the expressed-sequence-tag database (AA292123), and full-length hSmurf1 was reconstituted using a fragment of XSmurf1 cDNA encoding the first eight amino acids.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5B-Flag-Smurf1 wt was a gift from Jeff Wrana (Addgene plasmid # 11752)

  • For your References section:

    A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation. Zhu H, Kavsak P, Abdollah S, Wrana JL, Thomsen GH. Nature. 1999 Aug 12. 400(6745):687-93. 10.1038/23293 PubMed 10458166
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