pCMV5B-Flag-Smurf1 wt
(Plasmid #11752)

• Depositing Lab: Jeff Wrana
• Publication: Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Backbone

• Vector backbone: pCMV5B
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SMURF1
• Species: H. sapiens (human)
• Insert Size (bp): 2300
• GenBank ID: NM_020429
• Entrez Gene: SMURF1
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

A human Smurf1 cDNA encoding all but the first eight amino acids was identified in the expressed-sequence-tag database (AA292123), and full-length hSmurf1 was reconstituted using a fragment of XSmurf1 cDNA encoding the first eight amino acids.