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pcDNA3-hCdt1-HA
(Plasmid #117500)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117500 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3
  • Backbone manufacturer
    Invitrogene
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7128
  • Modifications to backbone
    HA tag
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cdc10-dependent transcript-1
  • Alt name
    Cdt1
  • Species
    H. sapiens (human); 1700 bp
  • Insert Size (bp)
    1700
  • Mutation
    wild-type
  • GenBank ID
    AB053172.1
  • Entrez Gene
    CDT1 (a.k.a. DUP, RIS2)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Source BioScience
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that mutation A262T in Cdt1 was found during Addgene's quality control. The depositor noted that this mutation should NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-hCdt1-HA was a gift from Domenico Maiorano (Addgene plasmid # 117500 ; http://n2t.net/addgene:117500 ; RRID:Addgene_117500)

  • For your References section:

    PIP degron proteins, substrates of CRL4Cdt2, and not PIP boxes, interfere with DNA polymerase eta and kappa focus formation on UV damage. Tsanov N, Kermi C, Coulombe P, Van der Laan S, Hodroj D, Maiorano D. Nucleic Acids Res. 2014 Apr;42(6):3692-706. doi: 10.1093/nar/gkt1400. Epub 2014 Jan 14. 10.1093/nar/gkt1400 PubMed 24423875
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