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Addgene

pYS2
(Plasmid #117490)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117490 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYS2
  • Vector type
    Deletion generation

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gfp, hyg
  • Species
    M. smegmatis

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene QC Next-generation sequencing identified a sequence discrepancy relative to the Genbank file linked under Supplemental Documents. Plasmid is expected to function as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYS2 was a gift from Eyal Gur (Addgene plasmid # 117490 ; http://n2t.net/addgene:117490 ; RRID:Addgene_117490)

  • For your References section:

    Efficient and simple generation of unmarked gene deletions in Mycobacterium smegmatis. Shenkerman Y, Elharar Y, Vishkautzan M, Gur E. Gene. 2014 Jan 1;533(1):374-8. doi: 10.1016/j.gene.2013.09.082. Epub 2013 Oct 4. 10.1016/j.gene.2013.09.082 PubMed 24100088
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