pSFFV-SpyTag-sfCherry2(11)-TagBFP
(Plasmid #117485)

• Purpose: Expresses tandem tag (SpyTag002-GG-sfCherry2(11) fused to N-terminal of TagBFP) in mammalian cells
• Depositing Lab: Bo Huang
• Publication: Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.1038/s42003-019-0589-x. eCollection 2019.

Backbone

• Vector backbone: pSFFV
• Backbone size w/o insert (bp): 9000
• Total vector size (bp): 9819
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SpyTag002-GG-sfCherry2(11)
• Species: Synthetic
• Insert Size (bp): 621
• Promoter: SFFV
• Tag / Fusion Protein:
  • TagBFP (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CGCGGGCCCGGGATCCATGgtgcctactatcgtgatggt
• 3′ sequencing primer: CTGGTGCAGGATTCGAGGTGCTGTGTCTGGCC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/454041v1 for BioRxiv preprint