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pSFFV-SpyTag-sfCherry2(11)-TagBFP
(Plasmid #117485)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117485 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSFFV
  • Backbone size w/o insert (bp) 9000
  • Total vector size (bp) 9819
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpyTag002-GG-sfCherry2(11)
  • Species
    Synthetic
  • Insert Size (bp)
    621
  • Promoter SFFV
  • Tag / Fusion Protein
    • TagBFP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGCGGGCCCGGGATCCATGgtgcctactatcgtgatggt
  • 3′ sequencing primer CTGGTGCAGGATTCGAGGTGCTGTGTCTGGCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/454041v1 for BioRxiv preprint

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSFFV-SpyTag-sfCherry2(11)-TagBFP was a gift from Bo Huang (Addgene plasmid # 117485 ; http://n2t.net/addgene:117485 ; RRID:Addgene_117485)

  • For your References section:

    Bright split red fluorescent proteins for the visualization of endogenous proteins and synapses. Feng S, Varshney A, Coto Villa D, Modavi C, Kohler J, Farah F, Zhou S, Ali N, Muller JD, Van Hoven MK, Huang B. Commun Biol. 2019 Sep 17;2:344. doi: 10.1038/s42003-019-0589-x. eCollection 2019. 10.1038/s42003-019-0589-x PubMed 31552297
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