Skip to main content
Addgene

pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)
(Plasmid #117484)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117484 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 6410
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpyCatcher002-6aa-sfCherry2(1-10)
  • Species
    Synthetic
  • Insert Size (bp)
    990
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TTAAACTTAAGCTTGggcgccatggtaaccacc
  • 3′ sequencing primer CTGGACTAGTGGATCCTCAGTCCTCGTTGTGGCTGGTGAT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/454041v1 for BioRxiv preprint

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10) was a gift from Bo Huang (Addgene plasmid # 117484 ; http://n2t.net/addgene:117484 ; RRID:Addgene_117484)

  • For your References section:

    Bright split red fluorescent proteins for the visualization of endogenous proteins and synapses. Feng S, Varshney A, Coto Villa D, Modavi C, Kohler J, Farah F, Zhou S, Ali N, Muller JD, Van Hoven MK, Huang B. Commun Biol. 2019 Sep 17;2:344. doi: 10.1038/s42003-019-0589-x. eCollection 2019. 10.1038/s42003-019-0589-x PubMed 31552297
Related items:
Commonly requested with: