pMO746
(Plasmid #117480)

• Purpose: upp in an artificial operon with npt from pMO9071 and AmpR-pUC ori from pCR4/TOPO, Pnpt-npt-upp;, KanR
• Depositing Lab: Judy Wall
• Publication: Parks et al Science. 2013 Mar 15;339(6125):1332-5. doi: 10.1126/science.1230667. Epub 2013 Feb 7.

Backbone

• Vector backbone: pCR4/TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 3205
• Modifications to backbone: pCR4/TOPO + DVU3152 (promoter and gene) => pMO731 || pMO9071 + upp (PCR product from D. vulgaris Hildenborough) => pMO745 || pUC and ampR (PCR product from pMO731) + kanR:upp (PCR product from pMO745) => pMO746
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: uracil phosphoribosyltransferase
• Alt name: upp
• Alt name: DVU1025
• Species: Desulfovibrio vulgaris
• Insert Size (bp): 627
• GenBank ID: NC_002937.3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Selection for kanamycin resistance after introduction of pMO746 identifies those cells in which integration into the chromosome of part or all of the plasmid has occurred.
Kanamycin resistance is a strong selection in the sulfate-reducing bacteria (SRB). Ampicillin is not universally effective in the SRB but is often used for selection in Escherichia coli. Thus this plasmid can be readily grown in E. coli but is actually unstable in the SRB.