Addgene: pCMV5B-Flag-Par6 wt Skip to main content
Addgene

pCMV5B-Flag-Par6 wt
(Plasmid #11748)

Full plasmid sequence is not available for this item.

Created with RaphaëlpCMV5B-Flag-Par6 wtXbaIPAR6FlagClaI

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11748 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pCMV5B
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PAR6
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1000
  • GenBank ID
    NM_019695
  • Entrez Gene
    Pard6a (a.k.a. 0710008C04Rik, 2610010A15Rik, PAR-6A, PAR6alpha, Par-6, Par6, Par6c, TAX40, Tip-40)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Tony Pawson
  • Article Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5B-Flag-Par6 wt was a gift from Jeff Wrana (Addgene plasmid # 11748 ; http://n2t.net/addgene:11748 ; RRID:Addgene_11748)
  • For your References section:

    Regulation of the polarity protein Par6 by TGFbeta receptors controls epithelial cell plasticity. Ozdamar B, Bose R, Barrios-Rodiles M, Wang HR, Zhang Y, Wrana JL. Science. 2005 Mar 11. 307(5715):1603-9. 10.1126/science.1105718 PubMed 15761148