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Addgene

pMO719
(Plasmid #117479)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117479 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR8/GW/TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 2817
  • Modifications to backbone
    captured pBG1 fragment
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Stable in Desulfovibrio (and other SRB strains). Selection in Desulfovibrio vulgaris Hildenborough is with 100 ug/ml spectinomycin.
  • Copy number
    High Copy

Cloning Information

  • Cloning method TOPO Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMO719 was a gift from Judy Wall (Addgene plasmid # 117479 ; http://n2t.net/addgene:117479 ; RRID:Addgene_117479)

  • For your References section:

    Development of a markerless genetic exchange system for Desulfovibrio vulgaris Hildenborough and its use in generating a strain with increased transformation efficiency. Keller KL, Bender KS, Wall JD. Appl Environ Microbiol. 2009 Dec;75(24):7682-91. doi: 10.1128/AEM.01839-09. Epub 2009 Oct 16. 10.1128/AEM.01839-09 PubMed 19837844
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