pSC27
(Plasmid
#117478)
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Purpose(Empty Backbone) mobilizable, SRB-stable, kanamycin-resistant cloning vector
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 117478 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTZ18U
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Backbone manufacturerYanisch-Perron et al.
- Backbone size (bp) 2860
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Modifications to backbonepTZ18U + pBG1 (EcoRI fragment) => pSC1 pSC1 + kanR (HindIII fragment from pUC4-KIXX) => pSC2 pSC2 + mob (DraII/EcoRV fragment from pJRD215) => pSC27
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsStable in Desulfovibrio (low copy number). Use G418 in place of kanamycin for some strains.
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSC27 was a gift from Judy Wall (Addgene plasmid # 117478 ; http://n2t.net/addgene:117478 ; RRID:Addgene_117478) -
For your References section:
New shuttle vectors for the introduction of cloned DNA in Desulfovibrio. Rousset M, Casalot L, Rapp-Giles BJ, Dermoun Z, de Philip P, Belaich JP, Wall JD. Plasmid. 1998;39(2):114-22. doi: 10.1006/plas.1997.1321. 10.1006/plas.1997.1321 PubMed 9514705
Map uploaded by the depositor.
