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Addgene

pHV738
(Plasmid #117476)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117476 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMON
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NMT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1488
  • Entrez Gene
    NMT1 (a.k.a. HsNMT1, NMT)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid also contains the gene that expresses E. coli methionine aminopeptidase under its own promoter

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHV738 was a gift from Richard Kahn (Addgene plasmid # 117476 ; http://n2t.net/addgene:117476 ; RRID:Addgene_117476)

  • For your References section:

    Coexpression of proteins with methionine aminopeptidase and/or N-myristoyltransferase in Escherichia coli to increase acylation and homogeneity of protein preparations. Van Valkenburgh HA, Kahn RA. Methods Enzymol. 2002;344:186-93. PubMed 11771383
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