pcDNA3-Myc-Smad8
(Plasmid #11745)

• Depositing Lab: Jeff Wrana
• Publication: Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-36.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SMAD8
• Species: R. norvegicus (rat)
• Insert Size (bp): 1600
• GenBank ID: AF012347
• Entrez Gene: Smad9 (a.k.a. Madh9, Smad8)
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: W. Vale, Salk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA