pCMV5-Smad5
(Plasmid #11744)

• Depositing Lab: Jeff Wrana
• Publication: Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-36.

Backbone

• Vector backbone: pCMV5
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SMAD5
• Species: M. musculus (mouse)
• Insert Size (bp): 1400
• GenBank ID: U58993
• Entrez Gene: Smad5 (a.k.a. 1110051M15Rik, Dwf-C, Madh5, MusMLP)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: J.M. Yingling, Duke
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA