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Addgene

Fly 2.0 pIDTBlue
(Plasmid #117418)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117418 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIDTBlue
  • Backbone manufacturer
    Integrated DNA Technologies
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 3005
  • Modifications to backbone
    pIDTBlue Ampicillin 5’ to T7 promoter
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FLY 2.0
  • Alt name
    FLY 2.0
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    75
  • Tag / Fusion Protein
    • NA

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer AGCGTTTTAAGCTCCAACGA
  • 3′ sequencing primer GTCCACTTGGGAGTCAGGAA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Fly 2.0 pIDTBlue was a gift from Dirk Dittmer (Addgene plasmid # 117418 ; http://n2t.net/addgene:117418 ; RRID:Addgene_117418)
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