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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11741 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepCMV5
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameALK2
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SpeciesH. sapiens (human)
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Insert Size (bp)1500
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GenBank IDNM_001105
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Entrez GeneACVR1 (a.k.a. ACTRI, ACVR1A, ACVRLK2, ALK2, FOP, SKR1, TSRI)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (destroyed during cloning)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV5-ALK2 wt was a gift from Jeff Wrana (Addgene plasmid # 11741 ; http://n2t.net/addgene:11741 ; RRID:Addgene_11741) -
For your References section:
Specific activation of Smad1 signaling pathways by the BMP7 type I receptor, ALK2. Macias-Silva M, Hoodless PA, Tang SJ, Buchwald M, Wrana JL. J Biol Chem. 1998 Oct 2. 273(40):25628-36. 10.1074/jbc.273.40.25628 PubMed 9748228