pAX2
(Plasmid #117398)

• Purpose: (Empty Backbone) allelic exchange vector with GFP merodiploid tracker, temperature-sensitive origin of replication, and TetR-controlled kill switch
• Depositing Lab: Karen Guillemin
• Publication: Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-18. doi: 10.1128/mBio.01877-18.

Backbone

• Vector backbone: pUC18R6K
• Modifications to backbone: A scaffold with interspersed restriction sites containing genes encoding chloramphenicol and gentamicin resistance and GFP was inserted into the backbone. In addition, the temperature-sensitive origin of replication ori101/repA101ts was inserted between the blunt restriction sites PsiI and SnaBI, destroying the R6K origin of replication. A synthetic kill switch consisting of a gene encoding the Tet repressor and a tet-controlled synthetic operon containing the genes hokB, ghoT, and tisB was also inserted into the backbone.
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: Also carries chloramphenicol and gentamicin selectable markers.
• Copy number: Low Copy

Resource Information

• Supplemental Documents:
  • pAX2.gbk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry