pTn7xKS
(Plasmid
#117393)
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Purpose(Empty Backbone) Tn7 tagging vector with a LacI-controlled kill switch counterselection system
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 117393 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC18
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Modifications to backboneA gene encoding the Lac repressor (lacIQ allele) and a lac-controlled synthetic operon containing three genes encoding the antibacterial toxins HokB, GhoT, and TisB were inserted into the backbone via blunt-ligation into a blunted NdeI restriction site.
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsAlso carries a gentamicin selectable marker
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTn7xKS was a gift from Karen Guillemin (Addgene plasmid # 117393 ; http://n2t.net/addgene:117393 ; RRID:Addgene_117393) -
For your References section:
Modernized Tools for Streamlined Genetic Manipulation and Comparative Study of Wild and Diverse Proteobacterial Lineages. Wiles TJ, Wall ES, Schlomann BH, Hay EA, Parthasarathy R, Guillemin K. MBio. 2018 Oct 9;9(5). pii: mBio.01877-18. doi: 10.1128/mBio.01877-18. 10.1128/mBio.01877-18 PubMed 30301859
