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Addgene

pCMV5B-FLAG-SARA dSBD
(Plasmid #11737)

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Item Catalog # Description Quantity Price (USD)
Plasmid 11737 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pCMV5B
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4000
  • Mutation
    deltaSBD: lacks Smad2-binding domain (aa665-704)
  • GenBank ID
    AF104304
  • Entrez Gene
    ZFYVE9 (a.k.a. MADHIP, NSP, PPP1R173, SARA, SMADIP)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5B-FLAG-SARA dSBD was a gift from Jeff Wrana (Addgene plasmid # 11737 ; http://n2t.net/addgene:11737 ; RRID:Addgene_11737)

  • For your References section:

    SARA, a FYVE domain protein that recruits Smad2 to the TGFbeta receptor. Tsukazaki T, Chiang TA, Davison AF, Attisano L, Wrana JL. Cell. 1998 Dec 11. 95(6):779-91. 10.1016/S0092-8674(00)81701-8 PubMed 9865696
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