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Addgene

pEZ-Frt-lox-DT
(Plasmid #11736)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11736 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBSII KS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Cre/Lox ; /Frt
  • Selectable markers
    Neomycin (select with G418) ; Diphtheriatoxin A

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pEZ-Frt-lox-DT
  • Species
    DNA derived from yeast, E. Coli, transposon Tn5, Cosynebacterium
  • Insert Size (bp)
    3100

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Cla I (not destroyed)
  • 3′ cloning site Don't Know (destroyed during cloning)
  • 5′ sequencing primer T7/M13
  • 3′ sequencing primer T7/M13
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Mark Ansel, CBR Institute for Biomedical Research
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

based on pEasyFlox. Vector for conditional gene targeting

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEZ-Frt-lox-DT was a gift from Klaus Rajewsky (Addgene plasmid # 11736 ; http://n2t.net/addgene:11736 ; RRID:Addgene_11736)
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