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Addgene

pCMV5-Smad7-HA
(Plasmid #11733)

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Item Catalog # Description Quantity Price (USD)
Plasmid 11733 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pCMV5
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SMAD7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • GenBank ID
    NM_005904
  • Entrez Gene
    SMAD7 (a.k.a. CRCS3, MADH7, MADH8)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5-Smad7-HA was a gift from Jeff Wrana (Addgene plasmid # 11733 ; http://n2t.net/addgene:11733 ; RRID:Addgene_11733)

  • For your References section:

    The MAD-related protein Smad7 associates with the TGFbeta receptor and functions as an antagonist of TGFbeta signaling. Hayashi H, Abdollah S, Qiu Y, Cai J, Xu YY, Grinnell BW, Richardson MA, Topper JN, Gimbrone MA, Wrana JL, Falb D. Cell. 1997 Jun 27. 89(7):1165-73. 10.1016/S0092-8674(00)80303-7 PubMed 9215638
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