pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1/2-5' Sequences (1)

Addgene Sequences:

Full (1)

Full Sequences from Addgene (1)

Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence).

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GenBank

SnapGene

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> Addgene NGS Result CTGCAGAATTGGCGCACGCGCTAAAAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAACA AAAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCT AGCTCTAAAACTGTTCACAGCGGACCTTGATCGGTGTTTCGTCCTTTCCACAAGATATATAAAGCCAAGA AATCGAAATACTTTCAAGTTACGGTAAGCATATGATAGTCCATTTTAAAACATAATTTTAAAACTGCAAA CTACCCAAGAAATTATTACTTTCTACGTCACGTATTTTGTACTAATATCTTTGTGTTTACAGTCAAATTA ATTCCAATTATCTCTCTAACAGCCTTGTATCGTATATGCAAATATGAAGGAATCATGGGAAATAGGCCCT CACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACT ATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACC GGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGC CTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACT GGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG GCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGG TAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACG CGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAA ATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGT GAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAA CTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGGCTTCCACGCTCACCGGC TCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCC GCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCA ACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGG TTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCT CCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTC TTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATA GTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACT TTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGAT CCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGG GTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTC ATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTG AATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTA AGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGT TTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGG ATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTA TGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATAAAATTGTAAACGTTAATATTTTGTTAAAATTC GCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGACCGAAATCGGCAAAATCCCTTATAAAT CAAAAGAATAGCCCGAGATAGAGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGT GGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCAAA TCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAG CTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAAGGC GCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGC GCGTACTATGGTTGCTTTGACGTATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCAT CAGGCGCCCCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGG GCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTA GGGGTTCCTGCGGCCGCTCCCCAGCATGCCTGCTATTCTCTTCCCAATCCTCCCCCTTGCTGTCCTGCCC CACCCCACCCCCCAGAATAGAATGACACCTACTCAGACAATGCGATGCAATTTCCTCATTTTATTAGGAA AGGACAGTGGGAGTGGCACCTTCCAGGGTCAAGGAAGGCACGGGGGAGGGGCAAACAACAGATGGCTGGC AACTAGAAGGCACAGTCGAGGCTGATCAGCGAGCTCTAGTTAGAATTCTCAGGCACCGGGCTTGCGGGTC ATGCACCAGGTGCGCGGTCCTTCGGGCACCTCGACGTCGGCGGTGACGGTGAAGCCGAGCCGCTCGTAGA AGGGGAGGTTGCGGGGCGCGGAGGTCTCCAGGAAGGCGGGCACCCCGGCGCGCTCGGCCGCCTCCACTCC GGGGAGCACGACGGCGCTGCCCAGACCCTTGCCCTGGTGGTCGGGCGAGACTCCGACGGTGGCCAGGAAC CACGCGGGCTCCTTGGGCCGGTGCGGCGCCAGGAGGCCTTCCATCTGTTGCTGCGCGGCCAGCCGGGAAC CGCTCAACTCGGCCATGCGCGGGCCGATCTCGGCGAACACCGCCCCCGCTTCGACGCTCTCCGGCGTGGT CCAGACCGCCACCGCGGCGCCGTCGTCCGCGACCCACACCTTGCCGATGTCGAGCCCGACGCGCGTGAGG AAGAGTTCTTGCAGCTCGGTGACCCGCTCGATGTGGCGGTCCGGATCGACGGTGTGGCGCGTGGCGGGGT AGTCGGCGAACGCGGCGGCGAGGGTGCGTACGGCCCTGGGGACGTCGTCGCGGGTGGCGAGGCGCACCGT GGGCTTGTACTCGGTCATTGGGCCAGGATTCTCCTCGACGTCACCGCATGTTAGCAGACTTCCTCTGCCC TCTCCACTGCCGAATTCCTTTTTCTTTTTTGCCTGGCCGGCCTTTTTCGTGGCCGCCGGCCTTTTGTCGC CTCCCAGCTGAGACAGGTCGATCCGTGTCTCGTACAGGCCGGTGATGCTCTGGTGGATCAGGGTGGCGTC CAGCACCTCTTTGGTGCTGGTGTACCTCTTCCGGTCGATGGTGGTGTCAAAGTACTTGAAGGCGGCAGGG GCTCCCAGATTGGTCAGGGTAAACAGGTGGATGATATTCTCGGCCTGCTCTCTGATGGGCTTATCCCGGT GCTTGTTGTAGGCGGACAGCACTTTGTCCAGATTAGCGTCGGCCAGGATCACTCTCTTGGAGAACTCGCT GATCTGCTCGATGATCTCGTCCAGGTAGTGCTTGTGCTGTTCCACAAACAGCTGTTTCTGCTCATTATCC TCGGGGGAGCCCTTCAGCTTCTCATAGTGGCTGGCCAGGTACAGGAAGTTCACATATTTGGAGGGCAGGG CCAGTTCGTTTCCCTTCTGCAGTTCGCCGGCAGAGGCCAGCATTCTCTTCCGGCCGTTTTCCAGCTCGAA CAGGGAGTACTTAGGCAGCTTGATGATCAGGTCCTTTTTCACTTCTTTGTAGCCCTTGGCTTCCAGAAAG TCGATGGGATTCTTCTCGAAGCTGCTTCTTTCCATGATGGTGATCCCCAGCAGCTCTTTCACACTCTTCA GTTTCTTGGACTTGCCCTTTTCCACTTTGGCCACCACCAGCACAGAATAGGCCACGGTGGGGCTGTCGAA GCCGCCGTACTTCTTAGGGTCCCAGTCCTTCTTTCTGGCGATCAGCTTATCGCTGTTCCTCTTGGGCAGG ATAGACTCTTTGCTGAAGCCGCCTGTCTGCACCTCGGTCTTTTTCACGATATTCACTTGGGGCATGCTCA GCACTTTCCGCACGGTGGCAAAATCCCGGCCCTTATCCCACACGATCTCCCCGGTTTCGCCGTTTGTCTC GATCAGAGGCCGCTTCCGGATCTCGCCGTTGGCCAGGGTAATCTCGGTCTTGAAAAAGTTCATGATGTTG CTGTAGAAGAAGTACTTGGCGGTAGCCTTGCCGATTTCCTGCTCGCTCTTGGCGATCATCTTCCGCACGT CGTACACCTTGTAGTCGCCGTACACGAACTCGCTTTCCAGCTTAGGGTACTTTTTGATCAGGGCGGTTCC CACGACGGCGTTCAGGTAGGCGTCGTGGGCGTGGTGGTAGTTGTTGATCTCGCGCACTTTGTAAAACTGG AAATCCTTCCGGAAATCGGACACCAGCTTGGACTTCAGGGTGATCACTTTCACTTCCCGGATCAGCTTGT CATTCTCGTCGTACTTAGTGTTCATCCGGGAGTCCAGGATCTGTGCCACGTGCTTTGTGATCTGCCGGGT TTCCACCAGCTGTCTCTTGATGAAGCCGGCCTTATCCAGTTCGCTCAGGCCGCCTCTCTCGGCCTTGGTC AGATTGTCGAACTTTCTCTGGGTAATCAGCTTGGCGTTCAGCAGCTGCCGCCAGTAGTTCTTCATCTTCT TCACGACCTCTTCGGAGGGCACGTTGTCGCTCTTGCCCCGGTTCTTGTCGCTTCTGGTCAGCACCTTGTT GTCGATGGAGTCGTCCTTCAGAAAGCTCTGAGGCACGATATGGTCCACATCGTAGTCGGACAGCCGGTTG ATGTCCAGTTCCTGGTCCACGTACATATCCCGCCCATTCTGCAGGTAGTACAGGTACAGCTTCTCGTTCT GCAGCTGGGTGTTTTCCACGGGGTGTTCTTTCAGGATCTGGCTGCCCAGCTCTTTGATGCCCTCTTCGAT CCGCTTCATTCTCTCGCGGCTGTTCTTCTGTCCCTTCTGGGTGGTCTGGTTCTCTCTGGCCATTTCGATC ACGATGTTCTCGGGCTTGTGCCGGCCCATCACTTTCACGAGCTCGTCCACCACCTTCACTGTCTGCAGGA TGCCCTTCTTAATGGCGGGGCTGCCGGCCAGATTGGCAATGTGCTCGTGCAGGCTATCGCCCTGGCCGGA CACCTGGGCTTTCTGGATGTCCTCTTTAAAGGTCAGGCTGTCGTCGTGGATCAGCTGCATGAAGTTTCTG TTGGCGAAGCCGTCGGACTTCAGGAAATCCAGGATTGTCTTGCCGGACTGCTTGTCCCGGATGCCGTTGA TCAGCTTCCGGCTCAGCCTGCCCCAGCCGGTGTATCTCCGCCGCTTCAGCTGCTTCATCACTTTGTCGTC GAACAGGTGGGCATAGGTTTTCAGCCGTTCCTCGATCATCTCTCTGTCCTCAAACAGTGTCAGGGTCAGC ACGATATCTTCCAGAATGTCCTCGTTTTCCTCATTGTCCAGGAAGTCCTTGTCCTTGATAATTTTCAGCA GATCGTGGTATGTGCCCAGGGAGGCGTTGAACCGATCTTCCACGCCGGAGATTTCCACGGAGTCGAAGCA CTCGATTTTCTTGAAGTAGTCCTCTTTCAGCTGCTTCACGGTCACTTTCCGGTTGGTCTTGAACAGCAGG TCCACGATGGCCTTTTTCTGCTCGCCGCTCAGGAAGGCGGGCTTTCTCATTCCCTCGGTCACGTATTTCA CTTTGGTCAGCTCGTTATACACGGTGAAGTACTCGTACAGCAGGCTGTGCTTGGGCAGCACCTTCTCGTT GGGCAGGTTCTTATCGAAGTTGGTCATCCGCTCGATGAAGCTCTGGGCGGAAGCGCCCTTGTCCACCACT TCCTCGAAGTTCCAGGGGGTGATGGTTTCCTCGCTCTTTCTGGTCATCCAGGCGAATCTGCTGTTTCCCC TGGCCAGAGGGCCCACGTAGTAGGGGATGCGGAAGGTCAGGATCTTCTCGATCTTTTCCCGGTTGTCCTT CAGGAATGGGTAAAAATCTTCCTGCCGCCGCAGAATGGCGTGCAGCTCTCCCAGGTGGATCTGGTGGGGG ATGCTGCCGTTGTCGAAGGTCCGCTGCTTCCGCAGCAGGTCCTCTCTGTTCAGCTTCACGAGCAGTTCCT CGGTGCCGTCCATCTTTTCCAGGATGGGCTTGATGAACTTGTAGAACTCTTCCTGGCTGGCTCCGCCGTC AATGTAGCCGGCGTAGCCGTTCTTGCTCTGGTCGAAGAAAATCTCTTTGTACTTCTCAGGCAGCTGCTGC CGCACGAGAGCTTTCAGCAGGGTCAGGTCCTGGTGGTGCTCGTCGTATCTCTTGATCATAGAGGCGCTCA GGGGGGCCTTGGTGATCTCGGTGTTCACTCTCAGGATGTCGCTCAGCAGGATGGCGTCGGACAGGTTCTT GGCGGCCAGAAACAGGTCGGCGTACTGGTCGCCGATCTGGGCCAGCAGGTTGTCCAGGTCGTCGTCGTAG GTGTCCTTGCTCAGCTGCAGTTTGGCATCCTCGGCCAGGTCGAAGTTGCTCTTGAAGTTGGGGGTCAGGC CCAGGCTCAGGGCAATCAGGTTTCCGAACAGGCCATTCTTCTTCTCGCCGGGCAGCTGGGCGATCAGATT TTCCAGCCGTCTGCTCTTGCTCAGTCTGGCAGACAGGATGGCCTTGGCGTCCACGCCGCTGGCGTTGATG GGGTTTTCCTCGAACAGCTGGTTGTAGGTCTGCACCAGCTGGATGAACAGCTTGTCCACGTCGCTGTTGT CGGGGTTCAGGTCGCCCTCGATCAGGAAGTGGCCCCGGAACTTGATCATGTGGGCCAGGGCCAGATAGAT CAGCCGCAGGTCGGCCTTGTCGGTGCTGTCCACCAGTTTCTTTCTCAGGTGGTAGATGGTGGGGTACTTC TCGTGGTAGGCCACCTCGTCCACGATGTTGCCGAAGATGGGGTGCCGCTCGTGCTTCTTATCCTCTTCCA CCAGGAAGGACTCTTCCAGTCTGTGGAAGAAGCTGTCGTCCACCTTGGCCATCTCGTTGCTGAAGATCTC TTGCAGATAGCAGATCCGGTTCTTCCGTCTGGTGTATCTTCTTCTGGCGGTTCTCTTCAGCCGGGTGGCC TCGGCTGTTTCGCCGCTGTCGAACAGCAGGGCTCCGATCAGGTTCTTCTTGATGCTGTGCCGGTCGGTGT TGCCCAGCACCTTGAATTTCTTGCTGGGCACCTTGTACTCGTCGGTGATCACGGCCCAGCCCACAGAGTT GGTGCCGATGTCCAGGCCGATGCTGTACTTCTTGTCGGCTGCTGGGACTCCGTGGATACCGACCTTCCGC TTCTTCTTTGGGGCCATCTTATCGTCATCGTCTTTGTAATCAATATCATGATCCTTGTAGTCTCCGTCGT GGTCCTTATAGTCCATGGTGGCACCGGTCCAACCTGAAAAAAAGTGATTTCAGGCAGGTGCTCCAGGTAA TTAAACATTAATACCCCACCAACCAACCATCCCTTAAACCCTTACCTCTTGCTCAGCTAATTACAGCCCG GAGGAGAAGGGCCGTCCCGCCCGCTCACCTGTGGGAGTAACGCGGTCAGTCAGAGCCGGGGCGGGCGGCG CGAGGCGGCGGCGGAGCGGGGCACGGGGCGAAGGCAGCGCGCAGCGACTCCCGCCCGCCGCGCGCTTCGC TTTTTATAGGGCCGCCGCCGCCGCCGCCTCGCCATAAAAGGAAACTTTCGGAGCGCGCCGCTCTGATTGG CTGCCGCCGCACCTCTCCGCCTCGCCCCGCCCCGCCCCTCGCCCCGCCCCGCCCCGCCTGGCGCGCGCCC CCCCCCCCCCCCGCCCCCATCGCTGCACAAAATAATTAAAAAATAAATAAATACAAAATTGGGGGTGGGG AGGGGGGGGAGATGGGGAGAGTGAAGCAGAACGTGGGGCTCACCTCGACCATGGTAATAGCGATGACTAA TACGTAGATGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGTACTGGGCACAATGCCAGGCGGGCCAT TTACCGTCATTGACGTCAATAGGGGGCGTACTTGGCATATGATACACTTGATGTACTGCCAAGTGGGCAG TTTACCGTAAATACTCCACCCATTGACGTCAATGGAAAGTCCCTATTGGCGTTACTATTGACGTCAATGG GCGGGGGTCGTTGGGCGGTCAGCCAGGCGGGCCATTTACCGTAAGTTATGTAACGGGTACCTCTAGAGCC ATTTGT

pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1/2-5' Sequences (1)

Full Sequences from Addgene (1)

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