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Addgene

RMC
(Plasmid #1173)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1173 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS316
  • Backbone size w/o insert (bp) 6018
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Rnq1-Sup35 fusion
  • Alt name
    Rnq1
  • Alt name
    Sup35
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2049
  • Mutation
    *(see below). Contains Rnq aa's 153-393 and Sup35 aa's 254-685(end)
  • Entrez Gene
    SUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)
  • Entrez Gene
    RNQ1 (a.k.a. YCL028W)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BanHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

*Note, Addgene's quality control sequencing finds S263P and N392T amino acid residue substitutions in the Rnq1 fragment. The effect of these residue changes is unknown.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RMC was a gift from Susan Lindquist (Addgene plasmid # 1173 ; http://n2t.net/addgene:1173 ; RRID:Addgene_1173)

  • For your References section:

    Rnq1: an epigenetic modifier of protein function in yeast. Sondheimer N, Lindquist S. Mol Cell 2000 Jan;5(1):163-72. 10.1016/S1097-2765(00)80412-8 PubMed 10678178
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