StrKDEL-IRES-ManII-mScarlet-i
(Plasmid #117274)

• Purpose: Expresses an Str-KDEL Hook for the RUSH system and separately mannosidase II-mScarlet-i
• Depositing Lab: David Stephens
• Publication: McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.201806035. doi: 10.1083/jcb.201806035.

Backbone

• Vector backbone: pIRESneo3
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: partial sequence of mannosidase II
• Species: M. musculus (mouse)
• Mutation: contains only amino acids 1 to 116 of ManII
• GenBank ID: NM_008549.2
• Entrez Gene: Man2a1 (a.k.a. Mana-2, Mana2, Map-2)
• Promoter: IRES
• Tag / Fusion Protein:
  • mScarlet-i (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: CMV

Gene/Insert 2

• Gene/Insert name: Streptavidin-KDEL
• Species: Synthetic
• Promoter: CMV

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: See PDF (unknown if destroyed)
• 3′ cloning site: See PDF (unknown if destroyed)
• 5′ sequencing primer: CMV

Resource Information

• Supplemental Documents:
  • Cloning information
• A portion of this plasmid was derived from a plasmid made by: Original clone was Plasmid #65253 from Franck Perez. mScarlet-i was synthesized from published sequence.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry