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PurposeExpresses Nfib and hygromycin resistance gene under control of tetO sequence.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 117271 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneTet-O-FUW
- Backbone size w/o insert (bp) 10013
- Total vector size (bp) 11276
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Vector typeMammalian Expression, Lentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNfib
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SpeciesM. musculus (mouse)
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Insert Size (bp)1263
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GenBank IDNM_008687
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Entrez GeneNfib (a.k.a. 6720429L07Rik, CTF, E030026I10Rik, NF-I/B, NF1-B, NFI-B)
- Promoter tet-on
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Tag
/ Fusion Protein
- IRES and hygromycin resistant cassette (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer LNCX
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
tetO.Nfib.Hygro was a gift from Henrik Ahlenius (Addgene plasmid # 117271 ; http://n2t.net/addgene:117271 ; RRID:Addgene_117271) -
For your References section:
Rapid and efficient induction of functional astrocytes from human pluripotent stem cells. Canals I, Ginisty A, Quist E, Timmerman R, Fritze J, Miskinyte G, Monni E, Hansen MG, Hidalgo I, Bryder D, Bengzon J, Ahlenius H. Nat Methods. 2018 Sep;15(9):693-696. doi: 10.1038/s41592-018-0103-2. Epub 2018 Aug 20. 10.1038/s41592-018-0103-2 [pii] PubMed 30127505