tetO.Sox9.Puro
(Plasmid #117269)

• Purpose: Expresses Sox9 and puromycin resistance gene under control of tetO sequence.
• Depositing Lab: Henrik Ahlenius
• Publication: Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi: 10.1038/s41592-018-0103-2. Epub 2018 Aug 20.

Backbone

• Vector backbone: Tet-O-FUW
• Backbone size w/o insert (bp): 9063
• Total vector size (bp): 10584
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sox9
• Species: M. musculus (mouse)
• Insert Size (bp): 1521
• GenBank ID: NM_011448
• Entrez Gene: Sox9 (a.k.a. 2010306G03Rik, mKIAA4243, mSox9)
• Promoter: tet-on
• Tag / Fusion Protein:
  • T2A and puromycin resistant cassette (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: WPRE-R

Resource Information

• Supplemental Documents:
  • tetO.Sox9.Puro.gb
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry