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MSCV-Bhlhe40-3xFLAG-IRES-BFP
(Plasmid #117263)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117263 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCV
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bhlhe40
  • Species
    M. musculus (mouse)
  • GenBank ID
  • Entrez Gene
    Bhlhe40 (a.k.a. Bhlhb2, C130042M06Rik, CR8, Clast5, Dec1, Sharp2, Stra13, Stra14)
  • Promoter LTR
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-Bhlhe40-3xFLAG-IRES-BFP was a gift from Sarah Teichmann (Addgene plasmid # 117263 ; http://n2t.net/addgene:117263 ; RRID:Addgene_117263)

  • For your References section:

    Genome-wide CRISPR Screens in T Helper Cells Reveal Pervasive Crosstalk between Activation and Differentiation. Henriksson J, Chen X, Gomes T, Ullah U, Meyer KB, Miragaia R, Duddy G, Pramanik J, Yusa K, Lahesmaa R, Teichmann SA. Cell. 2019 Jan 7. pii: S0092-8674(18)31569-1. doi: 10.1016/j.cell.2018.11.044. 10.1016/j.cell.2018.11.044 PubMed 30639098
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