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pLH02
(Plasmid #117262)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117262 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSIP411, pNZ5319
  • Vector type
    other

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 12.5 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PorfX-LVISKB_1732-30 , sapK-sappR
  • Alt name
    LVISKB_1732-30 (2065 bps), sapK-sappR (2095 bps)
  • Species
    Lactobacillus brevis ATCC367

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ApaI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Prof. Qingsheng Qi, State Key Laboratory of Microbial Technology, Shandong University, Jinan, China; plasmid: pLP-gba

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLH02 was a gift from Sheng Yang (Addgene plasmid # 117262 ; http://n2t.net/addgene:117262 ; RRID:Addgene_117262)

  • For your References section:

    Development of a RecE/T-Assisted CRISPR-Cas9 Toolbox for Lactobacillus. Huang H, Song X, Yang S. Biotechnol J. 2019 Jul;14(7):e1800690. doi: 10.1002/biot.201800690. Epub 2019 May 20. 10.1002/biot.201800690 PubMed 30927506
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