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Addgene

pHSB04X
(Plasmid #117258)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117258 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCas
  • Backbone manufacturer
    Sheng Yang, Addgene Plasmid #62225
  • Backbone size w/o insert (bp) 4476
  • Vector type
    other

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 25 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Cas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019
  • Alt name
    Cas9 (4107 bps), promoter PslpA-XbaI-guide-RNA (324 bps), homologous arm (1992 bps)
  • Species
    Cas9 (Streptococcus pyogenes M1 GAS)
  • GenBank ID
    Cas9:AE004092 (SPy_1046)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHSB04X was a gift from Sheng Yang (Addgene plasmid # 117258 ; http://n2t.net/addgene:117258 ; RRID:Addgene_117258)

  • For your References section:

    Development of a RecE/T-Assisted CRISPR-Cas9 Toolbox for Lactobacillus. Huang H, Song X, Yang S. Biotechnol J. 2019 Jul;14(7):e1800690. doi: 10.1002/biot.201800690. Epub 2019 May 20. 10.1002/biot.201800690 PubMed 30927506
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