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Addgene

pCS2-BRAT-EnR
(Plasmid #117252)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117252 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Vector type
    Xenopus expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BRAT-EnR
  • Alt name
    VegT-EnR
  • Alt name
    vegt.L L homeolog
  • Species
    X. laevis (frog)
  • Mutation
    chimeric protein with engrailed repressor domain (the first 298 amino acids of the Drosophila engrailed protein)
  • Entrez Gene
    vegt-a (a.k.a. brat, tVegT, xombi, antipodean)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-BRAT-EnR was a gift from Gerald Thomsen (Addgene plasmid # 117252 ; http://n2t.net/addgene:117252 ; RRID:Addgene_117252)

  • For your References section:

    A vegetally localized T-box transcription factor in Xenopus eggs specifies mesoderm and endoderm and is essential for embryonic mesoderm formation. Horb ME, Thomsen GH. Development. 1997 May;124(9):1689-98. PubMed 9165117
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