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Addgene

pEasy Flox
(Plasmid #11725)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11725 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBSII KS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Cre/Lox
  • Selectable markers
    Neomycin (select with G418) ; Thymidine kinase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pEasyFlox
  • Species
    DNA derived from yeast, Ecoli, transposon Tn5, Herpes Simplex
  • Insert Size (bp)
    3900

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7/NB
  • 3′ sequencing primer T7/M13
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Tri-loxP vector for conditional gene targeting

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEasy Flox was a gift from Klaus Rajewsky (Addgene plasmid # 11725 ; http://n2t.net/addgene:11725 ; RRID:Addgene_11725)

  • For your References section:

    DNA polymerase kappa deficiency does not affect somatic hypermutation in mice. Schenten D, Gerlach VL, Guo C, Velasco-Miguel S, Hladik CL, White CL, Friedberg EC, Rajewsky K, Esposito G. Eur J Immunol. 2002 Nov . 32(11):3152-60. 10.1002/1521-4141(200211)32:11<3152::AID-IMMU3152>3.0.CO;2-2 PubMed 12555660
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