pCS2-XTbx5-EnR
(Plasmid
#117249)
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PurposemRNA synthesis
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 117249 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+
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Vector typeXenopus expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTbx5-EnR
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SpeciesX. laevis (frog)
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Mutationchimeric protein with engrailed repressor domain (the first 298 amino acids of the Drosophila engrailed protein)
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Entrez Genetbx5-b (a.k.a. tbx-5, tbx5, xtbx-5, xtbx5)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2-XTbx5-EnR was a gift from Gerald Thomsen (Addgene plasmid # 117249 ; http://n2t.net/addgene:117249 ; RRID:Addgene_117249) -
For your References section:
Tbx5 is essential for heart development. Horb ME, Thomsen GH. Development. 1999 Apr;126(8):1739-51. PubMed 10079235