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Addgene

pCS2(+)-XSmurf1C699A
(Plasmid #117245)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117245 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Vector type
    Xenopus expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smurf1-C699A mutant
  • Species
    X. laevis (frog)
  • Mutation
    mutated cysteine 699 to alanine

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2(+)-XSmurf1C699A was a gift from Gerald Thomsen (Addgene plasmid # 117245 ; http://n2t.net/addgene:117245 ; RRID:Addgene_117245)

  • For your References section:

    A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation. Zhu H, Kavsak P, Abdollah S, Wrana JL, Thomsen GH. Nature. 1999 Aug 12. 400(6745):687-93. 10.1038/23293 PubMed 10458166
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