Tom20-CIB-GFP
(Plasmid #117242)

• Purpose: Light activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switch
• Depositing Lab: Robert Hughes
• Publication: Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16929. doi: 10.1074/jbc.RA119.009141. Epub 2019 Oct 3.

Backbone

• Vector backbone: phCMV-NGFP
• Backbone manufacturer: Genlantis
• Backbone size w/o insert (bp): 4962
• Total vector size (bp): 5733
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CIBN
• Alt name: Cryptochrome interacting bHLH N-terminus
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 771
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/551788v3 for bioRxiv preprint.