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Cry2L348F(1-531)-mCh-BAXS184E
(Plasmid #117239)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117239 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4018
  • Total vector size (bp) 6937
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cry2531.L348F-mCh-BAXS184E
  • Alt name
    Cryptochrome2L348F(1-531)-mCherry-BAXS184E
  • Species
    H. sapiens (human), A. thaliana (mustard weed)
  • Insert Size (bp)
    2919

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/551788v3 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cry2L348F(1-531)-mCh-BAXS184E was a gift from Robert Hughes (Addgene plasmid # 117239 ; http://n2t.net/addgene:117239 ; RRID:Addgene_117239)

  • For your References section:

    Imaging of morphological and biochemical hallmarks of apoptosis with optimized optogenetic tools. Godwin WC, Hoffmann GF, Gray TJ, Hughes RM. J Biol Chem. 2019 Nov 8;294(45):16918-16929. doi: 10.1074/jbc.RA119.009141. Epub 2019 Oct 3. 10.1074/jbc.RA119.009141 PubMed 31582560
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