Cry2(1-531)-mCh-BAXS184E
(Plasmid
#117238)
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PurposeLight activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switch
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 117238 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmCherry-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4018
- Total vector size (bp) 6937
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCry2531-mCh-BAXS184E
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Alt nameCryptochrome2(1-531)-mCherry-BAXS184E
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SpeciesH. sapiens (human), A. thaliana (mustard weed)
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Insert Size (bp)2919
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/551788v3 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Cry2(1-531)-mCh-BAXS184E was a gift from Robert Hughes (Addgene plasmid # 117238 ; http://n2t.net/addgene:117238 ; RRID:Addgene_117238) -
For your References section:
Imaging of morphological and biochemical hallmarks of apoptosis with optimized optogenetic tools. Godwin WC, Hoffmann GF, Gray TJ, Hughes RM. J Biol Chem. 2019 Nov 8;294(45):16918-16929. doi: 10.1074/jbc.RA119.009141. Epub 2019 Oct 3. 10.1074/jbc.RA119.009141 PubMed 31582560