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Addgene

U6a
(Plasmid #117221)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117221 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Piggybac
  • Total vector size (bp) 12610
  • Vector type
    Insect Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HR U6a promoter-gRNA
  • Alt name
    HR U6a-gRNA
  • gRNA/shRNA sequence
    white
  • Species
    Aedes aegypti
  • Promoter U6

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ttaattcgagctcgcccggggtcctaggCCTCACGCCACTCGTAGAATGAACCCGATT
  • 3′ sequencing primer CCAAAAACGACTTCAATCATTAGGggcgcgcccatactcggtggcctccccac
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    U6a was a gift from Omar Akbari (Addgene plasmid # 117221 ; http://n2t.net/addgene:117221 ; RRID:Addgene_117221)

  • For your References section:

    Development of a confinable gene drive system in the human disease vector Aedes aegypti. Li M, Yang T, Kandul NP, Bui M, Gamez S, Raban R, Bennett J, Sanchez C HM, Lanzaro GC, Schmidt H, Lee Y, Marshall JM, Akbari OS. Elife. 2020 Jan 21;9. pii: 51701. doi: 10.7554/eLife.51701. 10.7554/eLife.51701 PubMed 31960794
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