U6a
(Plasmid
#117221)
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PurposeExpresses gRNA in Aedes aegypti
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 117221 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePiggybac
- Total vector size (bp) 12610
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Vector typeInsect Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHR U6a promoter-gRNA
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Alt nameHR U6a-gRNA
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gRNA/shRNA sequencewhite
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SpeciesAedes aegypti
- Promoter U6
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ttaattcgagctcgcccggggtcctaggCCTCACGCCACTCGTAGAATGAACCCGATT
- 3′ sequencing primer CCAAAAACGACTTCAATCATTAGGggcgcgcccatactcggtggcctccccac (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
U6a was a gift from Omar Akbari (Addgene plasmid # 117221 ; http://n2t.net/addgene:117221 ; RRID:Addgene_117221) -
For your References section:
Development of a confinable gene drive system in the human disease vector Aedes aegypti. Li M, Yang T, Kandul NP, Bui M, Gamez S, Raban R, Bennett J, Sanchez C HM, Lanzaro GC, Schmidt H, Lee Y, Marshall JM, Akbari OS. Elife. 2020 Jan 21;9. pii: 51701. doi: 10.7554/eLife.51701. 10.7554/eLife.51701 PubMed 31960794