pEP PolI
(Plasmid #11722)

• Depositing Lab: Lawrence Loeb
• Publication: Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 100(17):9727-32.

Backbone

• Vector backbone: pHSG576
• Backbone manufacturer: see NCBI D88215
• Backbone size w/o insert (bp): 3475
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): JS200
• Growth instructions: Grow at 30oC in LB in JS200 cells (polA recA718). The cells need to be kept in exponential phase (do not let the culture reach saturation)!
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: DNA polymerase I EP
• Alt name: DNA polymerase I
• Alt name: PolA
• Alt name: PolI
• Species: E. coli
• Insert Size (bp): 2787
• Mutation: D424A, I709N, A759R. Error-prone version of PolI.
• Entrez Gene: polA (a.k.a. b3863, ECK3855, JW3835, resA)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: m13 reverse primer
• 3′ sequencing primer: m13 forward 20 primer

Resource Information

• Supplemental Documents:
  • Mutagenesis Protocol
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See attached mutagenesis protocol provided by depositing scientist.

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at or contact our distributors if you have any questions.