pAAV-FDIO-dAPEX2
(Plasmid #117175)

• Purpose: Peroxidase reporter for multiplexed electron microscopy labeling
• Depositing Lab: David Ginty
• Publication: Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/s41593-019-0358-7. doi: 10.1038/s41593-019-0358-7.

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 5651
• Total vector size (bp): 6404
• Vector type: Mammalian Expression, AAV ; Flp/FRT

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dAPEX2
• Species: G. max (soybean)
• Insert Size (bp): 753
• Mutation: W41F and A134P on soybean APX
• Promoter: EF1a

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: tcaagcctcagacagtggttc
• 3′ sequencing primer: CATAGCGTAAAAGGAGCAACA

Resource Information

• Supplemental Documents:
  • pAAV-FDIO-dAPEX2.gb
  • Staining Protocol

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry