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Addgene

Anep8_Cas9
(Plasmid #117169)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117169 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ANEp8
  • Backbone manufacturer
    Storms et al, 2005
  • Backbone size (bp) 11432
  • Modifications to backbone
    Insertion of Cas9 gene
  • Vector type
    CRISPR, Synthetic Biology ; Filamentous fungi expression
  • Promoter Pki

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Growth at 30 C for clones isolation
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer GCAGAATTTAATTAAATGGACAAGAAGTACTCCATTGGG
  • 3′ sequencing primer GCAGAATGGCCGGCCTTACACCTTCCTCTTCTTCTTGGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Anep8_Cas9 was a gift from Adrian Tsang (Addgene plasmid # 117169 ; http://n2t.net/addgene:117169 ; RRID:Addgene_117169)

  • For your References section:

    Efficient genome editing using tRNA promoter-driven CRISPR/Cas9 gRNA in Aspergillus niger. Song L, Ouedraogo JP, Kolbusz M, Nguyen TTM, Tsang A. PLoS One. 2018 Aug 24;13(8):e0202868. doi: 10.1371/journal.pone.0202868. eCollection 2018. PONE-D-18-15818 [pii] PubMed 30142205
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