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pASK-mSAN1-Streptag
(Plasmid #117165)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117165 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pASK-IBA3Plus
  • Backbone manufacturer
    IBA Lifesciences
  • Backbone size w/o insert (bp) 3172
  • Total vector size (bp) 5527
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grow at 18oC after induction with tetracycline
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mSAN1
  • Alt name
    CCPG; fam120b
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2355
  • GenBank ID
    NM_001357822.1
  • Entrez Gene
    Fam120b (a.k.a. 4932442K08Rik, AW049283, AW413338, CCPG, Pgcc1, mKIAA1838)
  • Promoter tetR/tetA
  • Tag / Fusion Protein
    • Streptag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bsa1 (not destroyed)
  • 3′ cloning site Bsa1 (not destroyed)
  • 5′ sequencing primer custom
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    MGC cDNA library

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

MGC cDNA was a partial clone, repaired by the authors to generate full length mSAN1. mSAN1 contains a difference compared to NM_001357822.1 that results in a V549I protein-coding change.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pASK-mSAN1-Streptag was a gift from Ian Macara (Addgene plasmid # 117165 ; http://n2t.net/addgene:117165 ; RRID:Addgene_117165)

  • For your References section:

    A senataxin-associated exonuclease SAN1 is required for resistance to DNA interstrand cross-links. Andrews AM, McCartney HJ, Errington TM, D'Andrea AD, Macara IG. Nat Commun. 2018 Jul 3;9(1):2592. doi: 10.1038/s41467-018-05008-8. 10.1038/s41467-018-05008-8 [pii] PubMed 29968717
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