pK-SAN1-Flag
(Plasmid #117163)

• Purpose: Expresses Flag-tagged SAN1 nuclease in mammalian cells.
• Depositing Lab: Ian Macara
• Publication: Andrews et al Nat Commun. 2018 Jul 3;9(1):2592. doi: 10.1038/s41467-018-05008-8.

Backbone

• Vector backbone: pRK7
• Backbone manufacturer: custom
• Backbone size w/o insert (bp): 4719
• Total vector size (bp): 7455
• Modifications to backbone: none
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: none

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SAN1
• Alt name: CCPG; fam120b
• Species: H. sapiens (human)
• Insert Size (bp): 2736
• GenBank ID: NM_001286379
• Entrez Gene: FAM120B (a.k.a. CCPG, KIAA1838, PGCC1, SAN1, dJ894D12.1)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xba1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: M13fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: NBRC, Japan

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

SAN1 contains a difference compared to the reference sequence NM_001286379 that results in a D370N difference in the protein product.