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Addgene

ScSpe1 pET-20b(+)
(Plasmid #117145)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117145 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-20b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3716
  • Total vector size (bp) 5114
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SPE1
  • Alt name
    Ornithine Decarboxylase
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1398
  • GenBank ID
    SPE1 (a.k.a. YKL184W) 853651
  • Promoter T7
  • Tag / Fusion Protein
    • Histidine (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ScSpe1 pET-20b(+) was a gift from Markus Ralser (Addgene plasmid # 117145 ; http://n2t.net/addgene:117145 ; RRID:Addgene_117145)

  • For your References section:

    Lysine harvesting is an antioxidant strategy and triggers underground polyamine metabolism. Olin-Sandoval V, Yu JSL, Miller-Fleming L, Alam MT, Kamrad S, Correia-Melo C, Haas R, Segal J, Pena Navarro DA, Herrera-Dominguez L, Mendez-Lucio O, Vowinckel J, Mulleder M, Ralser M. Nature. 2019 Aug;572(7768):249-253. doi: 10.1038/s41586-019-1442-6. Epub 2019 Jul 31. 10.1038/s41586-019-1442-6 PubMed 31367038
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