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pKLV2-U6(gDmap1 v2)-PGK-Puro-BFP
(Plasmid #117141)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117141 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W (Addgene: 67974)
  • Backbone size w/o insert (bp) 8629
  • Vector type
    Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gDmap1 v2
  • gRNA/shRNA sequence
    GAAGCGCAGATCAAATCGG
  • Species
    Synthetic
  • Insert Size (bp)
    19
  • Mutation
    Deleted BbsI site within WPRE, modified BFP
  • Entrez Gene
    Dmap1 (a.k.a. 1500016M21Rik, DNMAP1, DNMTAP1, Dmtap1, mKIAA1425)
  • Promoter human U6 promoter, mouse PGK promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKLV2-U6(gDmap1 v2)-PGK-Puro-BFP was a gift from Kosuke Yusa (Addgene plasmid # 117141 ; http://n2t.net/addgene:117141 ; RRID:Addgene_117141)

  • For your References section:

    CRISPR-KO screen identifies Dmap1 as a regulator of chemically-induced reprogramming and differentiation of cardiac progenitors. Yu JSL, Palano G, Lim C, Moggio A, Drowley L, Plowright AT, Bohlooly-Y M, Rosen BS, Hansson EM, Wang QD, Yusa K. Stem Cells. 2019 Apr 1. doi: 10.1002/stem.3012. 10.1002/stem.3012 PubMed 30932271
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