pKLV2-U6(BsmBI)-EF1a-BFP-BSD
(Plasmid #117136)

• Purpose: Empty vector encoding BFP-BSD
• Depositing Lab: Kosuke Yusa
• Publication: Yusa CRISPR-Cas9 and mammalian expression plasmids (unpublished)

Backbone

• Vector backbone: pKLV2-U6(BsmBI)-EF1a-BFP-Nkx2-5
• Backbone size w/o insert (bp): 9123
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BSD2A
• Species: Synthetic
• Insert Size (bp): 506
• Mutation: modified BFP
• Promoter: human U6 promoter, human EF1a promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)

Resource Information

• Supplemental Documents:
  • pKLV2-U6(BsmBI)-EF1aBFPBsd.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry